Review



α ncam 1  (R&D Systems)


Bioz Verified Symbol R&D Systems is a verified supplier
Bioz Manufacturer Symbol R&D Systems manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 95
      Buy from Supplier

    Structured Review

    R&D Systems α ncam 1
    α Ncam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/Human%2FMouse+NCAM-1%2FCD56+Antibody/pmc11394063-56-87-88
    Average 95 stars, based on 47 article reviews
    α ncam 1 - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein
    Article Snippet: Cells were incubated at 37 °C with 5% CO 2 and media was changed every 2–3 days.Cells were incubated at 37 °C with 5% CO 2 and media was changed every 2–3 days.. The antibodies used in this manuscript consisted of α-polySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgG-HRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).

    Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein.
    Article Snippet: Cells were incubated at 37 ◦C with 5% CO2 and media was changed every 2–3 days.Cells were incubated at 37 ◦C with 5% CO2 and media was changed every 2–3 days.. The antibodies used in this manuscript consisted of αpolySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgGHRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).

    Isolation:

    Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein
    Article Snippet: Cells were incubated at 37 °C with 5% CO 2 and media was changed every 2–3 days.Cells were incubated at 37 °C with 5% CO 2 and media was changed every 2–3 days.. The antibodies used in this manuscript consisted of α-polySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgG-HRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).

    Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein.
    Article Snippet: Cells were incubated at 37 ◦C with 5% CO2 and media was changed every 2–3 days.Cells were incubated at 37 ◦C with 5% CO2 and media was changed every 2–3 days.. The antibodies used in this manuscript consisted of αpolySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgGHRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).



    Similar Products

    fluorochrome-conjugated monoclonal antibodies α-cd56-pedazzle594  (Sony)
    90
    Sony fluorochrome-conjugated monoclonal antibodies α-cd56-pedazzle594
    Fluorochrome Conjugated Monoclonal Antibodies α Cd56 Pedazzle594, supplied by Sony, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/cd56+brilliant+violet+421+antibody/pm40499915-107-28-37
    Average 90 stars, based on 1 article reviews
    fluorochrome-conjugated monoclonal antibodies α-cd56-pedazzle594 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    α cd56 bv650  (Miltenyi Biotec)
    96
    Miltenyi Biotec α cd56 bv650
    α Cd56 Bv650, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/PSA-NCAM+Antibody%2C+anti-human%2Fmouse%2Frat/pm40394646-76-18-39
    Average 96 stars, based on 1 article reviews
    α cd56 bv650 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    cd271 pe hi100 557196 bd cd56 percpcy5 5 b159 560842 bd tnf α pe cy7 mab11 557647 bd cd107a apc h4a3  (Miltenyi Biotec)
    96
    Miltenyi Biotec cd271 pe hi100 557196 bd cd56 percpcy5 5 b159 560842 bd tnf α pe cy7 mab11 557647 bd cd107a apc h4a3
    Cd271 Pe Hi100 557196 Bd Cd56 Percpcy5 5 B159 560842 Bd Tnf α Pe Cy7 Mab11 557647 Bd Cd107a Apc H4a3, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/CD271+(LNGFR)+Antibody%2C+anti-human%2Fmouse%2C+Vio+Bright+FITC%2C+REAfinity/us12304962-486-17-36
    Average 96 stars, based on 1 article reviews
    cd271 pe hi100 557196 bd cd56 percpcy5 5 b159 560842 bd tnf α pe cy7 mab11 557647 bd cd107a apc h4a3 - by Bioz Stars, 2026-11
    96/100 stars
    		  Buy from Supplier
    α ncam 1  (R&D Systems)
    95
    R&D Systems α ncam 1
    α Ncam 1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/Human%2FMouse+NCAM-1%2FCD56+Antibody/pmc11394063-56-87-88
    Average 95 stars, based on 1 article reviews
    α ncam 1 - by Bioz Stars, 2026-11
    95/100 stars
    		  Buy from Supplier
    α-cd56  (Thermo Fisher)
    90
    Thermo Fisher α-cd56
    A. Representative IMC pseudoimages displaying NK cell-epithelial cell co-localization (αSMA = blue; E-cadherin, pan-cytokeratin = pink; NKG2D = yellow, scale bar = 100 µm). B. Representative multiplex immunofluorescence image of human PDAC showing NK cell-epithelial cell co-localization (n = 6; pan-cytokeratin = pink, <t>CD56</t> = yellow, NKG2D = cyan, scale bar = 50 µm). C. Cellular neighborhood analysis revealed 8 distinct cellular neighborhoods (red = high proportion of cells within cellular neighborhoods, blue = low proportion of cells within cellular neighborhoods). D. Cellular interaction analysis (blue = strong avoidance, red = strong interaction; FDR, P < 0.01) of IMC-defined cell subpopulations. E. Distance analysis per patient (n = 21) revealed that epithelial cells in PDAC are the closest to NK cells. Cell populations that were significantly distant from epithelial cells were calculated using 2-way ANOVA, Kruskal-Wallis test (* P < 0.05, *** P < 0.001, **** P < 0.0001;). F. Network graph of the top 75% of interactions between all IMC-defined cell populations in human PDAC showing epithelial cells and NK cells exist in similar networks.
    α Cd56, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/bio_rxiv__2024__05__23__593868-87-10-11
    Average 90 stars, based on 1 article reviews
    α-cd56 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier
    α cd56  (R&D Systems)
    90
    R&D Systems α cd56
    Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins <t>NCAM1</t> and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).
    α Cd56, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/%CE%B1+cd56/Human%2FPrimate+NCAM-1%2FCD56+Antibody/pmc11031136-160-13-14
    Average 90 stars, based on 1 article reviews
    α cd56 - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    A. Representative IMC pseudoimages displaying NK cell-epithelial cell co-localization (αSMA = blue; E-cadherin, pan-cytokeratin = pink; NKG2D = yellow, scale bar = 100 µm). B. Representative multiplex immunofluorescence image of human PDAC showing NK cell-epithelial cell co-localization (n = 6; pan-cytokeratin = pink, CD56 = yellow, NKG2D = cyan, scale bar = 50 µm). C. Cellular neighborhood analysis revealed 8 distinct cellular neighborhoods (red = high proportion of cells within cellular neighborhoods, blue = low proportion of cells within cellular neighborhoods). D. Cellular interaction analysis (blue = strong avoidance, red = strong interaction; FDR, P < 0.01) of IMC-defined cell subpopulations. E. Distance analysis per patient (n = 21) revealed that epithelial cells in PDAC are the closest to NK cells. Cell populations that were significantly distant from epithelial cells were calculated using 2-way ANOVA, Kruskal-Wallis test (* P < 0.05, *** P < 0.001, **** P < 0.0001;). F. Network graph of the top 75% of interactions between all IMC-defined cell populations in human PDAC showing epithelial cells and NK cells exist in similar networks.

    Journal: bioRxiv

    Article Title: Natural killer cells associate with epithelial cells in the pancreatic ductal adenocarcinoma tumor microenvironment

    doi: 10.1101/2024.05.23.593868

    Figure Lengend Snippet: A. Representative IMC pseudoimages displaying NK cell-epithelial cell co-localization (αSMA = blue; E-cadherin, pan-cytokeratin = pink; NKG2D = yellow, scale bar = 100 µm). B. Representative multiplex immunofluorescence image of human PDAC showing NK cell-epithelial cell co-localization (n = 6; pan-cytokeratin = pink, CD56 = yellow, NKG2D = cyan, scale bar = 50 µm). C. Cellular neighborhood analysis revealed 8 distinct cellular neighborhoods (red = high proportion of cells within cellular neighborhoods, blue = low proportion of cells within cellular neighborhoods). D. Cellular interaction analysis (blue = strong avoidance, red = strong interaction; FDR, P < 0.01) of IMC-defined cell subpopulations. E. Distance analysis per patient (n = 21) revealed that epithelial cells in PDAC are the closest to NK cells. Cell populations that were significantly distant from epithelial cells were calculated using 2-way ANOVA, Kruskal-Wallis test (* P < 0.05, *** P < 0.001, **** P < 0.0001;). F. Network graph of the top 75% of interactions between all IMC-defined cell populations in human PDAC showing epithelial cells and NK cells exist in similar networks.

    Article Snippet: Samples were incubated with α-pan-cytokeratin-eFluor™ 660 (Invitrogen, CAT#: 50-9003-82) and α-CD56 (Invitrogen, CAT#: MA1-19129) antibodies overnight (25°C).

    Techniques: Multiplex Assay, Immunofluorescence

    A. Schematic for Transwell invasion assays. Average relative invasion + SEM of B. Donor NK #1 (n = 9), C. Donor NK #2 (n = 12), D. Donor NK #3 (n = 6), and E. Donor NK #4 (n = 10) upon CD44 neutralization. * P < 0.05 as determined by Wilcoxon matched-pairs signed rank test. F. Schematic of spheroid invasion assay. G. Representative 20X immunofluorescence images of outlined (yellow) PANC-1 spheroids (pan-cytokeratin; red) embedded with NK cells (CD56; green), pointed out with white arrows, treated with or without α-CD44. Scale bar = 250 µm. H. Zoomed inset of NK cells in the α-CD44 treatment group in G (white box). Average number of NK cells per PANC-1 spheroid + SEM from I. Donor NK #1 (- α-CD44, n = 42; + α-CD44, n = 53), J. Donor NK #2 (- α-CD44, n = 43; + α-CD44, n = 29), and K. Donor NK #3 (- α-CD44, n = 47; + α-CD44, n = 27); n = # of spheroids analyzed; * P < 0.05 as determined by Wilcoxon matched-pairs signed rank test.

    Journal: bioRxiv

    Article Title: Natural killer cells associate with epithelial cells in the pancreatic ductal adenocarcinoma tumor microenvironment

    doi: 10.1101/2024.05.23.593868

    Figure Lengend Snippet: A. Schematic for Transwell invasion assays. Average relative invasion + SEM of B. Donor NK #1 (n = 9), C. Donor NK #2 (n = 12), D. Donor NK #3 (n = 6), and E. Donor NK #4 (n = 10) upon CD44 neutralization. * P < 0.05 as determined by Wilcoxon matched-pairs signed rank test. F. Schematic of spheroid invasion assay. G. Representative 20X immunofluorescence images of outlined (yellow) PANC-1 spheroids (pan-cytokeratin; red) embedded with NK cells (CD56; green), pointed out with white arrows, treated with or without α-CD44. Scale bar = 250 µm. H. Zoomed inset of NK cells in the α-CD44 treatment group in G (white box). Average number of NK cells per PANC-1 spheroid + SEM from I. Donor NK #1 (- α-CD44, n = 42; + α-CD44, n = 53), J. Donor NK #2 (- α-CD44, n = 43; + α-CD44, n = 29), and K. Donor NK #3 (- α-CD44, n = 47; + α-CD44, n = 27); n = # of spheroids analyzed; * P < 0.05 as determined by Wilcoxon matched-pairs signed rank test.

    Article Snippet: Samples were incubated with α-pan-cytokeratin-eFluor™ 660 (Invitrogen, CAT#: 50-9003-82) and α-CD56 (Invitrogen, CAT#: MA1-19129) antibodies overnight (25°C).

    Techniques: Neutralization, Invasion Assay, Immunofluorescence

    Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins NCAM1 and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).

    Journal: Glycobiology

    Article Title: Site-specific immobilization of the endosialidase reveals QSOX2 is a novel polysialylated protein

    doi: 10.1093/glycob/cwae026

    Figure Lengend Snippet: Proteomic analysis of polysialylated proteins from NK-92 cells. A) Immunoblot analysis of NK-92 cell lysate samples treated with PBS or with active EndoN before isolation on immobilized EndoN DM . α-polySia and α-NCAM blots show lysate (L), flowthrough (F), wash (W), and boiled beads (B). B) Volcano plot of proteins identified in samples relative to those pretreated with active EndoN. Proteins with a significant difference are labelled. The known polysialylated proteins NCAM1 and ST8Sia4 are highlighted ( n = 4 per group, assessed with student’s T-test).

    Article Snippet: The antibodies used in this manuscript consisted of α-polySia mAb 735 (BioAspect BA-Ab00240-2.0), α-CD56 (R&D systems, mAB2408), α-beta-tubulin (Abcam, ab6046), streptavidin-HRP (Biolegend, 405210), goat α-mouse IgG-HRP (R&D systems, HAF007), goat α-rabbit IgG-HRP (Abcam, ab6721), and α-QSOX2 (Abcam, ab191168 and ab121376).

    Techniques: Western Blot, Isolation